Journal Article

Determination of Acetonitrile and Cyanide in Rat Blood: Application to an Experimental Study

Fabiano Henrique Mateus, José Salvador Lepera and Vera Lucia Lanchote

in Journal of Analytical Toxicology

Volume 29, issue 2, pages 105-109
Published in print March 2005 | ISSN: 0146-4760
Published online March 2005 | e-ISSN: 1945-2403 | DOI: http://dx.doi.org/10.1093/jat/29.2.105
Determination of Acetonitrile and Cyanide in Rat Blood: Application to an Experimental Study

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Methods were developed for the analysis of acetonitrile and its metabolite cyanide in the blood of rats exposed to acetonitrile. Acetonitrile was analyzed by the headspace technique coupled to gas chromatography with detection by flame ionization, and cyanide was analyzed by high-performance liquid chromatography with fluorescence detection (λex = 418 nm and λem = 460 nm) after derivatization of the ion with naphthalene 2,3-dicarboxyaldehyde and taurine. The quantitation limits of the methods for the analysis of acetonitrile and cyanide were 4.875 µg/mL and 0.025 µg/mL, respectively. The coefficients of variation of 10% or less obtained for intra- and interassay precision indicate the precision of these analytical methods and the systematic errors, all less than 5%, indicate that the methods are quite accurate. The methods were applied to an experimental study after the animals received acetonitrile at the doses of 2 mmol/kg or 5 mmol/kg.

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Subjects: Medical Toxicology ; Toxicology (Non-medical)

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