Journal Article

Urinary Excretion of α-Hydroxytriazolam Following a Single Dose of Halcion<sup>®</sup>

Dong-Liang Lin, Tsun-Ying Huang, Hsiu-Chuan Liu and Rea-Ming Yin

in Journal of Analytical Toxicology

Volume 29, issue 2, pages 118-123
Published in print March 2005 | ISSN: 0146-4760
Published online March 2005 | e-ISSN: 1945-2403 | DOI: http://dx.doi.org/10.1093/jat/29.2.118
Urinary Excretion of α-Hydroxytriazolam Following a Single Dose of Halcion®

More Like This

Show all results sharing these subjects:

  • Medical Toxicology
  • Toxicology (Non-medical)

GO

Show Summary Details

Preview

As an approved medicinal product and reportedly an abused substance that have been associated with death and “considered to be a factor…of impaired driving, sexual assault, and other violent crimes”, triazolam is controlled at the same level (Level III) as flunitrazepam in Taiwan. Alleged misuses of this substance have been associated with case specimens submitted to this laboratory. A sample preparation (with and without enzymatic hydrolysis) and gas chromatography-mass spectrometry protocols were evaluated and applied to the analysis of free and total α-hydroxytriazolam (the main metabolite of triazolam) in urine. Ions designated for TMS-derivatized α-hydroxytriazolam and α-hydroxytriazolam-d4 are m/z 415, 417, and 430 and 419, 421, and 434, respectively. The overall protocol achieved the following results when applied to the analysis of 2-mL drug-free urine specimens fortified with 10–200 ng/mL α-hydroxytriazolam: recovery, 95%; interday and intraday precision ranges, 1.50–3.52% and 0.93–4.71%, respectively; linearity, r2 > 0.99; and limits of detection and quantitation, 0.05 and 0.1 ng/mL, respectively. This protocol was applied to the analysis of case specimens and urine samples collected from two patients (A and B) taking one oral dose of Halcion (0.25 mg triazolam). Excretion profiles of free and total α-hydroxytriazolam show that free α-hydroxytriazolam is detectable, but at very low levels (< 5 ng/mL). Peak excretion of total α-hydroxytriazolam occurs at approximately 5–10 h following the drug intake. Total α-hydroxytriazolam is excreted at detectable levels approximately 2–35 h following an oral dose of 0.25 mg triazolam. Total free and conjugated α-hydroxytriazolam excreted by A and B are 0.61% and 31.6%; and 0.36% and 57.2% of the dose, respectively.

Journal Article.  0 words. 

Subjects: Medical Toxicology ; Toxicology (Non-medical)

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.