Journal Article

A New GC-MS Method for the Determination of Five Amphetamines in Human Hair

J.L. Villamor, A.M. Bermejo, P. Fernández and M.J. Tabernero

in Journal of Analytical Toxicology

Volume 29, issue 2, pages 135-139
Published in print March 2005 | ISSN: 0146-4760
Published online March 2005 | e-ISSN: 1945-2403 | DOI: http://dx.doi.org/10.1093/jat/29.2.135
A New GC-MS Method for the Determination of Five Amphetamines in Human Hair

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A new gas chromatography-mass spectrometry method for the simultaneous identification and quantitation of amphetamine (AP), methamphetamine (MA), 3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxymethamphetamine (MDMA), and 3,4-methylenedioxyethamphetamine (MDEA) in hair is proposed. Hair was hydrolyzed in 1M NaOH at 40°C subjected to extraction with 4:1 (v/v) methylene chloride/isopropanol, and derivatized with pentafluoropropionic anhydride (PFPA) and ethyl acetate. Calibration curves for the five analytes were constructed over the concentration range 0.5–25.0 ng/mg, using their pentadeuterated analogues as internal standards. The limits of detection and quantitation obtained were 0.045 and 0.151 ng/mg for AP; 0.014 and 0.048 ng/mg for MA; 0.013 and 0.043 ng/mg for MDA; 0.017 and 0.057 ng/mg for MDMA; and 0.007 and 0.023 ng/mg for MDEA. The accuracy of the method was found to be in the range ± 9%, and the coefficients of variation were less than 8%. Overall, 24 hair specimens tested positive for one or more amphetamines, with average concentrations of 0.88 ng/mg for AP, 10.14 ng/mg for MA, 1.30 ng/mg for MDA, and 8.87 ng/mg for MDMA. Only one specimen tested positive for MDEA with a concentration of 0.84 ng/mg.

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Subjects: Medical Toxicology ; Toxicology (Non-medical)

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