Journal Article

Simple Liquid Chromatographic Method for the Rapid and Simultaneous Determination of Propoxur and its Major Metabolite Isopropoxy Phenol in Rat Blood and Urine Using Solid-Phase Extraction

Ramagiri Suma, R.K Sarin, P.K. Saiprakash and Sistla Ramakrishna

in Journal of Analytical Toxicology

Volume 29, issue 7, pages 728-733
Published in print October 2005 | ISSN: 0146-4760
Published online October 2005 | e-ISSN: 1945-2403 | DOI: http://dx.doi.org/10.1093/jat/29.7.728
Simple Liquid Chromatographic Method for the Rapid and Simultaneous Determination of Propoxur and its Major Metabolite Isopropoxy Phenol in Rat Blood and Urine Using Solid-Phase Extraction

More Like This

Show all results sharing these subjects:

  • Medical Toxicology
  • Toxicology (Non-medical)

GO

Show Summary Details

Preview

This research paper describes the development and validation of an analytical method for the simultaneous determination of propoxur and isopropoxy phenol (IPP, a major metabolite) in both blood and urine of rat using reversed-phase high-performance liquid chromatography (HPLC) employing solid-phase extraction (SPE). Sample purification was performed using a weak cation-exchange cartridge (Isolute® CBA). Separation was achieved by HPLC with UV detection at 270 nm. Recoveries of propoxur and IPP from blood and urine by SPE exceeded 85%. The validated calibration range for propoxur is from 0.5 to 100 µg/L and 2 to 100 µg/L for IPP in both rat blood and urine. The limit of quantitation for propoxur in blood and urine is 0.5 and 0.8 µg/L, respectively, and 2.0 and 4.2 µg/L, respectively, for IPP. Validation results on specificity, sensitivity, linearity, precision, accuracy, and stability are shown. The applicability of the method was demonstrated by the analysis of urine and blood from rats that were orally fed propoxur at minimum dose.

Journal Article.  0 words. 

Subjects: Medical Toxicology ; Toxicology (Non-medical)

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.