Journal Article

High-Performance Liquid Chromatography Method for Urinary <i>trans, trans</i>-Muconic Acid. Application to Environmental Exposure to Benzene

Valentina Olmos, Silvia C. Lenzken, Clara M. López and Edda C. Villaamil

in Journal of Analytical Toxicology

Volume 30, issue 4, pages 258-261
Published in print May 2006 | ISSN: 0146-4760
Published online May 2006 | e-ISSN: 1945-2403 | DOI: http://dx.doi.org/10.1093/jat/30.4.258
High-Performance Liquid Chromatography Method for Urinary trans, trans-Muconic Acid. Application to Environmental Exposure to Benzene

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This report describes a specific and precise high-performance liquid chromatography (HPLC) method for the quantification of trans, trans-muconic acid in human urine. The procedure involved a highly efficient Bond-Elut SAX extraction with 20% acetic acid elution. The HPLC analysis used a sodium acetate/methanol mobile phase with a C18 reverse phase column and UV detection at 265 nm. The recovery, precision, linearity, and limits of detection and quantification of the method were determined. Mean absolute recoveries were between 97% and 115 %. The calibration curve showed a correlation coefficient of 0.9955 and the limit of detection was determined to be 10.8 µg/L. The method is suitable for evaluation of occupational and environmental benzene exposure in humans. The study of urinary trans, trans-muconic acid of two populations of children to evaluate environmental benzene exposure is presented.

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Subjects: Medical Toxicology ; Toxicology (Non-medical)

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