Journal Article

The Detection of Δ<sup>9</sup>-Tetrahydrocannabinol (THC) and 11-nor-9-Carboxy-Δ<sup>9</sup>-Tetrahydrocannabinol (THCA) in Whole Blood Using Two-Dimensional Gas Chromatography and El-Mass Spectrometry

Rodger D. Scurlock, Greg B. Ohlson and David K. Worthen

in Journal of Analytical Toxicology

Volume 30, issue 4, pages 262-266
Published in print May 2006 | ISSN: 0146-4760
Published online May 2006 | e-ISSN: 1945-2403 | DOI: http://dx.doi.org/10.1093/jat/30.4.262
The Detection of Δ9-Tetrahydrocannabinol (THC) and 11-nor-9-Carboxy-Δ9-Tetrahydrocannabinol (THCA) in Whole Blood Using Two-Dimensional Gas Chromatography and El-Mass Spectrometry

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A method is described for the simultaneous analysis of Δ9-tetrahydrocannabinol (THC) and its carboxylic acid metabolite, 11-nor-9-carboxy-Δ9-tetrahydrocannabinol (THCA) as their trimethylsiyl derivatives using 2-dimensional chromatography and electron ionization-mass spectrometric detection. The addition of a Deans switch to a standard GC oven allows the use of two chromatographic columns of differing stationary phase to greatly reduce matrix interference. The analytes are extracted from 1 mL of whole blood by first precipitating the blood proteins with the addition of acetonitrile followed by solid-phase extraction. The limit of quantitation for both THC and THCA was determined to be 1.0 ng/mL. The between-run precision at 1.0 ng/mL (N = 30) was 7.7% and 7.4% for THC and THCA, respectively. The method is linear from 1 to 100 ng/mL.

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Subjects: Medical Toxicology ; Toxicology (Non-medical)

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