Journal Article

Measurement of Benzylmercapturic Acid in Human Urine by Liquid Chromatography-Electrospray Ionization-Tandem Quadrupole Mass Spectrometry

Jui-Shu Chou, Yi-Chang Lin, Yee-Chung Ma, Jenn-Feng Sheen and Tung-Sheng Shih

in Journal of Analytical Toxicology

Volume 30, issue 5, pages 306-312
Published in print June 2006 | ISSN: 0146-4760
Published online June 2006 | e-ISSN: 1945-2403 | DOI: http://dx.doi.org/10.1093/jat/30.5.306
Measurement of Benzylmercapturic Acid in Human Urine by Liquid Chromatography-Electrospray Ionization-Tandem Quadrupole Mass Spectrometry

More Like This

Show all results sharing these subjects:

  • Medical Toxicology
  • Toxicology (Non-medical)

GO

Show Summary Details

Preview

A method based on liquid chromatography (reversed-phase)-electrospray (negative) ionization-tandem triple quadrupole mass spectrometry [LC-ESI(-)-MS-MS], in the multiple reaction monitoring mode, was developed for determination of urinary benzylmercapturic acid (BMA). Isotope dilution through introduction of 13C6-labeled BMA was employed as an internal standard, achieving intra- and interrun precision ranges of 2.32–2.95% and 2.24–4.97%, respectively. The calibration curve obtained with BMA-spiked blank human urine was linear from 0.5–120.0 µg/L; the correlation coefficient of the calibration curve was 0.999. The method limit of quantification was 0.5 µg/L; mean BMA recovery was 97.56%. The analytical method was used to analyze urine samples collected from workers involved in the manufacture of adhesive tape who had been exposed to toluene. The method appears to provide sensitive, specific, and reliable testing for urinary BMA to determine occupational exposure to toluene.

Journal Article.  0 words. 

Subjects: Medical Toxicology ; Toxicology (Non-medical)

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.