Journal Article

Stability Study of Propoxur (Baygon) in Whole Blood and Urine Stored at Varying Temperature Conditions

Suma Ramagiri, Hari Kosanam and P.K. Sai Prakash

in Journal of Analytical Toxicology

Volume 30, issue 5, pages 313-316
Published in print June 2006 | ISSN: 0146-4760
Published online June 2006 | e-ISSN: 1945-2403 | DOI: http://dx.doi.org/10.1093/jat/30.5.313
Stability Study of Propoxur (Baygon) in Whole Blood and Urine Stored at Varying Temperature Conditions

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A stability study has been initiated for propoxur (Baygon) in whole blood and urine samples stored over a period of 60 days at four different temperature conditions (room temperature, 4°C, −20°C, and −80°C). Stability data was established on day 0, 1, 7, 14, 28, 42, and 60. Sample purification was done by solid-phase extraction using a weak cation exchange cartridge (Isolute® CBA), and quantitation was carried out by a validated high-performance liquid chromatographic method with a photodiode-array UV detector. Propoxur was spiked at two different concentration levels in both blood and urine samples [low concentration (10 µg/L) and high concentration (100 µg/L)]. Isopropoxy phenol was observed as the major degradation product in blood and urine samples and confirmed by liquid chromatography-electrospray ionization-mass spectrometry. At room temperature, a substantial decrease in concentration of about 95% was observed at the end of the stability study in both blood and urine samples. However, at 4°C, the concentration of propoxur observed after 60 days was around 60% in both samples. A decrease in temperature reduced the degradation, and finally propoxur was found to be stable at −80°C and −20°C for the whole observation period (60 days). The data collected suggests that knowledge about time-dependent decrease of propoxur in urine and blood samples is of considerable significance in forensic toxicology, and, therefore, forensic cases should be interpreted with caution.

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Subjects: Medical Toxicology ; Toxicology (Non-medical)

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