Journal Article

Solid-Phase Column Chromatographic and Gas Chromatographic-Mass Spectrometric Determination of Heptaminol in Human Urine and Related Pharmacokinetic Profiles

Ying Lung Tseng, Chin-Hung Liu, Fan-Hsin Kuo and Min-Hua Shieh

in Journal of Analytical Toxicology

Volume 30, issue 6, pages 365-369
Published in print July 2006 | ISSN: 0146-4760
Published online July 2006 | e-ISSN: 1945-2403 | DOI: http://dx.doi.org/10.1093/jat/30.6.365
Solid-Phase Column Chromatographic and Gas Chromatographic-Mass Spectrometric Determination of Heptaminol in Human Urine and Related Pharmacokinetic Profiles

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Heptaminol is an antihypotensive drug and is one of the stimulants banned in sport competitions. When heptaminol was fortified to a drug-free urine sample and subjected to solid-phase extraction, trifluroacetic anhydride derivatization, and gas chromatography-mass spectrometry analysis, the results indicated three chromatographic peaks, with one major peak [peak 1 (P1) as heptaminol-2TFA], appearing at retention time 7.17 min, and two minor peaks [peak 2 (P2) andpeak 3 (P3) as heptaminol-TFA], appearing at RT 5.87 and 5.81 min, respectively. The characteristic ions of peak mass spectra were m/z 322, 224, and 140 for P1, m/z 223 (molecular ion), 208, 140, and 110 for P2, and m/z 208, 140, and 110 for P3. The urine samples collected from healthy male volunteers who orally ingested a single dose (100 mg) of heptaminol were similar to the analytical results shown in the heptaminol-spiked control urine samples. This result suggested that the unchanged heptaminol was the sole form found inurine. The unchanged parent compound was completely eliminated inurine within 24 h and an average of approximately 97% of the dose was excreted through the renal pathway.

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Subjects: Medical Toxicology ; Toxicology (Non-medical)

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