Journal Article

The Quantitation of Procaine in Equine Plasma by Liquid Chromatography-Linear Ion Trap Mass Spectrometry

Keith D. Zientek, Danielle F. Anderson, Kirsten Wegner and Cynthia Cole

in Journal of Analytical Toxicology

Volume 31, issue 2, pages 87-92
Published in print March 2007 | ISSN: 0146-4760
Published online March 2007 | e-ISSN: 1945-2403 | DOI: http://dx.doi.org/10.1093/jat/31.2.87
The Quantitation of Procaine in Equine Plasma by Liquid Chromatography-Linear Ion Trap Mass Spectrometry

More Like This

Show all results sharing these subjects:

  • Medical Toxicology
  • Toxicology (Non-medical)

GO

Show Summary Details

Preview

A method for the extraction and quantitation of procaine in equine plasma was developed for use with liquid chromatography-mass spectrometry (LC-MS). Procaine was isolated from equine plasma by liquid-liquid extraction at pH 11 with dichloromethane using procaine-d10 as an internal standard. Quantitation was achieved by LC-MS using a 3-µm C-18 column coupled to an electrospray ionization source on a linear ion-trap mass spectrometer. The limit of detection and limit of quantitation was determined to be 50 and 200 pg/mL, respectively. The lowest limit of detection determined by previous methods was 1 ng/mL. Administration samples were obtained as part of a larger study to determine a regulatory limit for procaine in racehorses and procaine concentrations were determined using this method.

Journal Article.  0 words. 

Subjects: Medical Toxicology ; Toxicology (Non-medical)

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.