Journal Article

Rapid Quantification of Buprenorphine-Glucuronide and Norbuprenorphine-Glucuronide in Human Urine by LC-MS-MS

S. Hegstad, H.Z. Khiabani, E.L. Øiestad, T. Berg and A.S. Christophersen

in Journal of Analytical Toxicology

Volume 31, issue 4, pages 214-219
Published in print May 2007 | ISSN: 0146-4760
Published online May 2007 | e-ISSN: 1945-2403 | DOI: http://dx.doi.org/10.1093/jat/31.4.214
Rapid Quantification of Buprenorphine-Glucuronide and Norbuprenorphine-Glucuronide in Human Urine by LC-MS-MS

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A liquid chromatography-tandem mass spectrometry (LC-MS-MS) method was developed and validated for the determination of buprenorphine-glucuronide (BUP-G) and norbuprenorphineglucuronide (NBUP-G) in human urine. The method included a dilution step followed by filtration through a Mini-Uniprep Filter and direct injection onto the LC column. The analytes were quantified in multiple reactions monitoring mode using one transition ion. Norbuprenorpine-d3 (NBUP-d3) was used as the internal standard. The concentration ranges were 6–161 ng/mL for BUP-G and 12–295 ng/mL for NBUP-G. Recoveries determined after filtration for the analytes were 75%. The between-day precision of the method was in the range of 4.8–11%. The limits of quantification were found to be 4.6 ng/mL for BUP-G and 11.8 ng/mL for NBUP-G. Approximately 1000 samples from law enforcement, prison inmates, probation services, and hospitals were analyzed by the presented method. The ratios of drug glucuronides versus creatinine were calculated for a selection of samples (n = 151), where there was information on treatment with buprenorphine between 16 and 20 rag/day. The majority (86%) of the samples had a ratio of BUP-G/creatinine below 570 µg/g, and 76% of the samples had NBUP-G/creatinine lower than 1060 µg/g. The LC-MS-MS method proved to be robust and specific for the determination of BUP-G and NBUP-G in urine.

Journal Article.  0 words. 

Subjects: Medical Toxicology ; Toxicology (Non-medical)

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