Journal Article

Microwave-Assisted Extraction and HPLC-DAD Determination of Drugs of Abuse in Human Plasma

Purificación Fernáindez, Marta Lago, Rosa Antonia Lorenzo, Antonia María Carro, Ana María Bermejo and María Jesús Tabernero

in Journal of Analytical Toxicology

Volume 31, issue 7, pages 388-393
Published in print September 2007 | ISSN: 0146-4760
Published online September 2007 | e-ISSN: 1945-2403 | DOI: http://dx.doi.org/10.1093/jat/31.7.388
Microwave-Assisted Extraction and HPLC-DAD Determination of Drugs of Abuse in Human Plasma

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A sample preparation procedure using microwave energy is proposed for the determination of morphine, 6-acetylmorphine, codeine, cocaine, cocaethylene, benzoylecgonine, methadone, and 2-ethylene-1,5-dimethyl-3,3-diphenylpyrrolidine in human plasma. A screening asymmetrical factorial design was used to identify the most suitable extraction conditions as regards solvent, temperature, and extraction time. The target drugs were quantified by high-performance liquid chromatography with diode-array detection. The use of microwave energy was found to reduce solvent consumption and extraction time compared with solid-phase extraction. The detector response was linear over the drug concentration range of 0.05–2.0 µg/mL in human plasma. The precision and accuracy were good, with values less than 8% and 7%, respectively. Drug recoveries from spiked samples ranged from 69 to 81%. The proposed method was successfully applied to a number of forensic cases.

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Subjects: Medical Toxicology ; Toxicology (Non-medical)

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