Journal Article

Determination of Lovastatin Hydroxy Acid in Female B6C3F<sub>1</sub> Mouse Serum

Jon W. Lodge, Brenda L. Fletcher, Sherri S. Brown, Angela J. Parham, Reshan A. Fernando and Bradley J. Collins

in Journal of Analytical Toxicology

Volume 32, issue 3, pages 248-252
Published in print April 2008 | ISSN: 0146-4760
Published online April 2008 | e-ISSN: 1945-2403 | DOI: http://dx.doi.org/10.1093/jat/32.3.248
Determination of Lovastatin Hydroxy Acid in Female B6C3F1 Mouse Serum

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A liquid chromatographic-mass spectrometricmethod for the determination of lovastatin hydroxy acid in female B6C3F1 mouse serum was developed for use in supporting toxicokinetic studies of animals dosed with the cholesterol lowering agent lovastatin. The method does not require an extensive sample cleanup and shows good correlation between serum matrix standards and solvent standards. The method was validated and used to analyze serum samples from a preliminary dose level range-finding study. The method was validated for a concentration range of approximately 1.0 to 100 ng/mL in serum, and linearity was verified to ∼2000 ng/mL The stability of sample extracts was determined under various storage conditions and the stability of serum samples stored frozen was determined over a period of seven weeks. During the course of analyzing the animal samples, the serum was monitored for the presence of lovastatin not hydrolyzed to the hydroxy acid, but no attempt was made to quantify lovastatin. No unhydrolyzed lovastatin was noted in any of the serum samples from animals dosed with lovastatin.

Journal Article.  0 words. 

Subjects: Medical Toxicology ; Toxicology (Non-medical)

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