Journal Article

Determination of <i>p-tert</i>-Octylphenol in Blood and Tissues by Gas Chromatography Coupled with Mass Spectrometry

G. Hamelin, G. Charest-Tardif, K. Krishnan, D.G. Cyr, M. Charbonneau, P.J. Devine, S. Haddad, G.M. Cooke, T. Schrader and R. Tardif

in Journal of Analytical Toxicology

Volume 32, issue 4, pages 303-307
Published in print May 2008 | ISSN: 0146-4760
Published online May 2008 | e-ISSN: 1945-2403 | DOI: http://dx.doi.org/10.1093/jat/32.4.303
Determination of p-tert-Octylphenol in Blood and Tissues by Gas Chromatography Coupled with Mass Spectrometry

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A sensitive and reproducible procedure using gas chromatography coupled with mass spectrometry is described for the determination of p-tert-octylphenol (OP), a persistent degradation product of alkylphenol ethoxylates that binds to the estrogen receptor in blood and tissues. The first step involved the extraction of blood (200 µL) or tissue homogenate (400 µL) with methyl tert-butyl ether, including p-tert-butylphenol (BP) as internal standard. After extraction, the sample was evaporated to dryness with a gentle stream of nitrogen at 45°C, and OP and BP were derivatized with an acetylation reaction involving acetic anhydride and catalyzed by pyridine. Samples were then analyzed by a gas chromatograph equipped with a mass spectrometer (single ion monitoring) with a Varian VF-5ms capillary column. The limit of detection and the limit of quantification of the method in blood were 4.6 and 15.5 ng/mL, respectively. The linearity and reproducibility of the method were acceptable, with coefficients of variation of approximately 10% for blood and ranging between 9% and 27% for tissues. This method was applied to the determination of unchanged OP in blood and tissues obtained from Sprague-Dawley rats after oral and IV OP administration.

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Subjects: Medical Toxicology ; Toxicology (Non-medical)

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