Journal Article

A Gas Chromatography-Isotope Dilution High-Resolution Mass Spectrometry Method for Quantification of Isomeric Benzo[a]pyrene Diol Epoxide Hemoglobin Adducts in Humans

Angela D. Ragin, Kenroy E. Crawford, Alisha A. Etheredge, James Grainger and Donald G. Patterson

in Journal of Analytical Toxicology

Volume 32, issue 9, pages 728-736
Published in print November 2008 | ISSN: 0146-4760
Published online November 2008 | e-ISSN: 1945-2403 | DOI: http://dx.doi.org/10.1093/jat/32.9.728
A Gas Chromatography-Isotope Dilution High-Resolution Mass Spectrometry Method for Quantification of Isomeric Benzo[a]pyrene Diol Epoxide Hemoglobin Adducts in Humans

More Like This

Show all results sharing these subjects:

  • Medical Toxicology
  • Toxicology (Non-medical)

GO

Show Summary Details

Preview

We developed a gas chromatography-isotope dilution high-resolution mass spectrometry (GC-ID-HRMS) method for quantifying isomers of benzo[a]pyrene (BaP) tetrol metabolites resulting from hydrolysis of benzo[a]pyrene-diol-epoxide hemoglobin (BaPDE-Hb) adducts. Acid hydrolysis of BPDE-Hb adducts extracted from human blood samples yielded isomers of benzo[a]pyrene-tetrahydrotetrols, (±)-BaP-r-7,t-8,t-9,c-10-tetrol (BPTI-1), (±)-BaP-r-7,t-8,t-9,t-10-tetrol (BPTI-2), (±)-BaP-r-7,t-8,c-9,t-10-tetrol (BPTII-1), and (±)-BaP-r-7,t-8,c-9,c-10-tetrol (BPTII-2). The isomeric BaP tetrols were isolated from the matrix by liquid-liquid extraction, and then further purified by solid-phase extraction. Following silylation with N-methyl-N-(trimethylsilyl)-trifluoroacetamide, the analytes were measured by GC-HRMS, using electron ionization. We have found detectable concentrations in the low fmol range for BPTII-1 and BPTI-1 in all donors tested. The mean BaP adduct levels for smoking donors (n = 9) were 0.022 fmol/mg hemoglobin for BPTII-1 and 0.070 fmol/mg hemoglobin for BPTI-1. The mean BaP adduct levels with hemoglobin for non smoking donors (n = 6) was 0.021 fmol/mg hemoglobin for BPTII-1 and 0.105 fmol/mg hemoglobin for BPTI-1.

Journal Article.  0 words. 

Subjects: Medical Toxicology ; Toxicology (Non-medical)

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.