Journal Article

Determination of Ethylglucuronide in Oral Fluid by Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry

S. Hegstad, L. Johnsen, J. Mørland and A.S. Christophersen

in Journal of Analytical Toxicology

Volume 33, issue 4, pages 204-207
Published in print May 2009 | ISSN: 0146-4760
Published online May 2009 | e-ISSN: 1945-2403 | DOI: http://dx.doi.org/10.1093/jat/33.4.204
Determination of Ethylglucuronide in Oral Fluid by Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry

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An ultra-performance liquid chromatography-tandem mass spectrometry method has been developed and validated for the determination of ethylglucuronide (EtG) in oral fluid. Sample clean-up was achieved by solid-phase extraction with a Hyper-SEP SAX column. Negative ionization was performed in the multiple reaction monitoring mode. Two transitions were monitored for the analyte and one for the internal standard EtG-d5. The calibration range was 4.4–222 ng/mL. The recovery of the analyte ranged from 86 to 99%, and the between-assay precisions ranged from 5 to 9% RSD. The limit of quantification was found to be 4.4 ng/mL. The concentration of EtG in oral fluid collected 2–14 h after a moderate alcohol intake varied from 13.3 to 57.7 ng/mL.

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Subjects: Medical Toxicology ; Toxicology (Non-medical)

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