Journal Article

Development and Validation of a High-Performance Liquid Chromatography—Tandem Mass Spectrometry Method for the Rapid Simultaneous Quantification of Aconitine, Mesaconitine, and Hypaconitine in Rat Plasma after Oral Administration of Sini Decoction

Lei-ping He, Bin Di, Ying-xiang Du, Fang Yan, Meng-xiang Su, Hua-qing Liu and Lin-jun You

in Journal of Analytical Toxicology

Volume 33, issue 9, pages 588-594
Published in print November 2009 | ISSN: 0146-4760
Published online November 2009 | e-ISSN: 1945-2403 | DOI: http://dx.doi.org/10.1093/jat/33.9.588
Development and Validation of a High-Performance Liquid Chromatography—Tandem Mass Spectrometry Method for the Rapid Simultaneous Quantification of Aconitine, Mesaconitine, and Hypaconitine in Rat Plasma after Oral Administration of Sini Decoction

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A rapid, sensitive, and specific liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS-MS) method was developed and validated for simultaneous determination of aconitine (AC), mesaconitine (MA), and hypaconitine (HA), the three toxic constituents from Sini decoction (SND) in rat plasma. After the addition of citalopram as the internal standard (IS), plasma samples were basified with 100 µL 10% ammonium hydroxide, and then extracted with 1 mL ethyl acetate. Chromatographic separation was performed on a CN column (250 mm × 4.6 mm, 5 µm) with a mobile phase of methanol/40 mM ammonium acetate/formic acid (950:45:5, v/v/v) at the flow rate of 1.0 mL/min. Analytes were determined in a triple-quadrupole mass spectrometer in the selected reaction-monitoring (SRM) mode using electrospray source with positive mode. The method was validated over the concentration ranges of 0.01–10 ng/mL for AC, MA, and HA. The variation coefficients were always < 15% for both intraday and interday precision for each analyte. Mean accuracies were also within ±15%. The method was proved to be sensitive, rapid, specific, accurate, and reproducible. It has been successfully applied to the pharmacokinetics study on rats after oral administration of SND.

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Subjects: Medical Toxicology ; Toxicology (Non-medical)

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