Journal Article

Analysis of Tetrahydrocannabinol and its Metabolite, 11-Nor-Δ<sup>9</sup>-Tetrahydrocannabinol-9-Carboxylic Acid, in Oral Fluid using Liquid Chromatography with Tandem Mass Spectrometry

Cynthia Coulter, Margaux Garnier and Christine Moore

in Journal of Analytical Toxicology

Volume 36, issue 6, pages 413-417
Published in print July 2012 | ISSN: 0146-4760
Published online April 2012 | e-ISSN: 1945-2403 | DOI:
Analysis of Tetrahydrocannabinol and its Metabolite, 11-Nor-Δ9-Tetrahydrocannabinol-9-Carboxylic Acid, in Oral Fluid using Liquid Chromatography with Tandem Mass Spectrometry

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This paper describes the determination of tetrahydrocannabinol (THC) and its metabolite, 11-nor-Δ9-tetrahydrocannabinol-9-carboxylic acid (THC-COOH) in oral fluid using solid-phase extraction and liquid chromatography with tandem mass spectral detection (LC–MS-MS) and its application to proficiency specimens. The method employs collection of oral fluid with the Quantisal™ device, base hydrolysis, solid-phase extraction and LC–MS-MS in positive ion electrospray mode. Because the concentration of the metabolite in oral fluid is quite low, extremely sensitive analytical methods are necessary. The requisite sensitivity was achieved by a simple, rapid derivatization of the compound after extraction. The derivatization conditions did not affect parent THC. The method was fully validated using standard parameters including linearity, sensitivity, accuracy, intra-day and inter-day imprecision, drug recovery from the collection pad, limit of quantitation, limit of detection and matrix effects. The procedure was applied to oral fluid proficiency specimens previously analyzed to assess the stability of THC-COOH.

Journal Article.  3037 words.  Illustrated.

Subjects: Medical Toxicology ; Toxicology (Non-medical)

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