Journal Article

Evaluation of Buprenorphine LUCIO Immunoassay versus GC–MS Using Urines from a Workplace Drug Testing Program

Marcela Nogueira Rabelo Alves, Alberto Piccinotti, Silvia Tameni and Aldo Polettini

in Journal of Analytical Toxicology

Volume 37, issue 3, pages 175-178
Published in print April 2013 | ISSN: 0146-4760
Published online February 2013 | e-ISSN: 1945-2403 | DOI: http://dx.doi.org/10.1093/jat/bkt006
Evaluation of Buprenorphine LUCIO Immunoassay versus GC–MS Using Urines from a Workplace Drug Testing Program

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The buprenorphine (BUP) LUCIO Nal Von Minden screening assay was evaluated. Urine samples from subjects enrolled in a workplace drug testing program were screened according to the manufacturer's instruction using a Roche COBAS Integra 800 analyser. For gas chromatography–mass spectrometry (GC–MS) confirmatory analysis, samples were submitted to enzymatic hydrolysis with β-glucuronidase and mixed-mode solid-phase extraction. Imprecision (coefficient of variation) for 3.0, 7.0, and 13.0 ng/mL calibrators varied within 2.8–8.7 intra-day (n = 20) and 7.7–8.6 inter-day (n = 19). Inaccuracy (bias) was between –5.6–30.5 intra-day and –13.2–4.2 inter-day. At the 5 ng/mL cut-off, the immunoassay showed 100% sensitivity and 88% specificity, with an overall agreement of 94% between immunoassay and GC–MS. Raising the cut-off to 10 ng/mL provided an identical overall agreement between immunoassay and GC–MS (94%), despite the decrease in sensitivity (90%) and the increase in specificity (100%). According to these results, the BUP LUCIO Nal Von Minden screening assay provides adequate sensitivity and specificity for BUP screening in urine samples using a cut-off concentration of 5 ng/mL.

Journal Article.  2387 words. 

Subjects: Medical Toxicology ; Toxicology (Non-medical)

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