Journal Article

Quantification of Polybrominated and Polychlorinated Biphenyls in Human Matrices by Isotope-Dilution Gas Chromatography–Tandem Mass Spectrometry

M. Elizabeth Marder, Parinya Panuwet, Ronald E. Hunter, P. Barry Ryan, Michele Marcus and Dana Boyd Barr

in Journal of Analytical Toxicology

Volume 40, issue 7, pages 511-518
Published in print September 2016 | ISSN: 0146-4760
Published online August 2016 | e-ISSN: 1945-2403 | DOI: http://dx.doi.org/10.1093/jat/bkw041
Quantification of Polybrominated and Polychlorinated Biphenyls in Human Matrices by Isotope-Dilution Gas Chromatography–Tandem Mass Spectrometry

More Like This

Show all results sharing these subjects:

  • Medical Toxicology
  • Chemistry
  • Toxicology (Non-medical)

GO

Show Summary Details

Preview

We have developed a highly sensitive and selective analytical method capable of quantifying a total of 15 polybrominated and polychlorinated biphenyls (11 PBBs and 4 PCBs) in human serum. Samples were subjected to liquid–liquid extraction followed by solid-phase extraction prior to measurement using gas chromatography–tandem mass spectrometry in multiple reaction monitoring mode. Quantification was performed using isotope-dilution calibration covering a concentration range of 0.005–12.5 ng/mL. Limits of detection for all target compounds were in the low range (0.7–6.5 pg/mL). The method was validated using in-house pooled human serum fortified at two concentrations (0.5 ng/mL and 1.0 ng/mL), whole semen fortified at one concentration (0.25 ng/mL), and NIST Standard Reference Material (SRM) 1958, which includes five of the target compounds. Method accuracies for all target compounds ranged from 84 to 119% with relative standard deviations (RSDs) of <19%. The measured values for the five target compounds present in the SRM agreed with the certified reference values (89–119% accuracy with RSDs <9%). As this method was developed to support ongoing epidemiologic investigations, we evaluated its suitability by analyzing subsets of serum and whole semen samples from the Michigan PBB Registry cohort. PBB-153, PCB-118, PCB-138, PCB-153 and PCB-180 were detected in all serum samples analyzed, with PBB-77 and PBB-101 detected less frequently in serum. PBB-153, PCB-118, PCB-138, PCB-153 and PCB-180 were detected in at least one whole semen sample.

Journal Article.  5367 words.  Illustrated.

Subjects: Medical Toxicology ; Chemistry ; Toxicology (Non-medical)

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.