Journal Article

Comparison of human blood concentrations of collectin kidney 1 and mannan-binding lectin

Takayuki Yoshizaki, Katsuki Ohtani, Wataru Motomura, Seong-Jae Jang, Ken-ichiro Mori, Noritoshi Kitamoto, Itsuro Yoshida, Yasuhiko Suzuki and Nobutaka Wakamiya

in The Journal of Biochemistry

Published on behalf of The Japanese Biochemical Society

Volume 151, issue 1, pages 57-64
Published in print January 2012 | ISSN: 0021-924X
Published online September 2011 | e-ISSN: 1756-2651 | DOI: http://dx.doi.org/10.1093/jb/mvr114
Comparison of human blood concentrations of collectin kidney 1 and mannan-binding lectin

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Mannan-binding lectin (MBL) was first discovered as a collectin in animal blood, and was shown to have such unique characteristics as a collage-like domain and a carbohydrate recognition domain. We recently identified human collectin kidney 1 (CL-K1, COLEC11) from a human kidney cDNA library. To quantitate the CL-K1 concentration in blood, we developed several polyclonal and monoclonal antibodies using recombinant human CL-K1 in CHO cells and the CL-K1 fragment in Escherichia coli. Using these antibodies, we established a sandwich enzyme-linked immunosorbent assay (ELISA) system. The concentration of CL-K1 in human plasma was 0.34 ± 0.13 µg/ml and that in MBL was 1.72 ± 1.51 µg/ml. Concentrations of MBL are often low due to its single nucleotide polymorphisms (SNPs) which seem to be related to an opsonic defect. However, no low concentrations of CL-K1 were observed on testing over two hundred blood samples. We also found that the blood concentration of CL-K1 was not dependent on gender or age and did not correlate completely with that of MBL. The ELISA system developed in this study will be useful for elucidating the physiological and pathophysiological role of CL-K1 in humans.

Keywords: complement; blood coagulation; lectin; carbohydrate; genetic

Journal Article.  3538 words.  Illustrated.

Subjects: Biochemistry

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