Journal Article

The monoclonal antibody MAC252 does not react with the (−) enantiomer of abscisic acid

Philippe Barrieu and Thierry Simonneau

in Journal of Experimental Botany

Published on behalf of Society for Experimental Biology

Volume 51, issue 343, pages 305-307
Published in print February 2000 | ISSN: 0022-0957
Published online February 2000 | e-ISSN: 1460-2431 | DOI: http://dx.doi.org/10.1093/jexbot/51.343.305
The monoclonal antibody MAC252 does not react with the (−) enantiomer of abscisic acid

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An RIA procedure has been developed for ABA quantification using MAC62, a monoclonal antibody raised against (+)‐cis, trans‐ABA. This widely used method now relies on MAC252, a recloned version of the exhausted MAC62. Recently, it has been suggested that MAC252 was not able to discriminate between the (+) and (−) enantiomers of ABA. As this can be misleading when interpreting RIA results, it has been carefully examined here whether MAC252 reacts with (−)‐ABA. MAC252 exhibited negligible cross‐reactivity with (−)‐ABA, which was confirmed with commercial mixtures of ABA isomers. It is concluded that the RIA protocol can continue to be used with MAC252 as it was with MAC62.

Keywords: RIA; MAC252; ABA isomers

Journal Article.  1926 words.  Illustrated.

Subjects: Plant Sciences and Forestry

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