Journal Article

Chlorophyll fluorescence—a practical guide

Kate Maxwell and Giles N. Johnson

in Journal of Experimental Botany

Volume 51, issue 345, pages 659-668
Published in print April 2000 | ISSN: 0022-0957
Published online April 2000 | e-ISSN: 1460-2431 | DOI: http://dx.doi.org/10.1093/jexbot/51.345.659
Chlorophyll fluorescence—a practical guide

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Chlorophyll fluorescence analysis has become one of the most powerful and widely used techniques available to plant physiologists and ecophysiologists. This review aims to provide an introduction for the novice into the methodology and applications of chlorophyll fluorescence. After a brief introduction into the theoretical background of the technique, the methodology and some of the technical pitfalls that can be encountered are explained. A selection of examples is then used to illustrate the types of information that fluorescence can provide.

Keywords: Chlorophyll fluorescence; electron transport; photoinhibition.; Fv /Fm, ratio of variable to maximum fluorescence—the quantum efficiency of open photosystem II centres; Fm, maximum fluorescence yield; Fo, minimum fluorescence yield; Fs or Ft, steady‐state fluorescence yield; J, photosynthetic electron transport rate (μequiv m−2 s−1); NPQ, non‐photochemical quenching; PFD, photon flux density (400–700 nm); PSII, photosystem II, qP, photochemical quenching; qE, energy‐dependent quenching; qI, photoinhibitory quenching; qT, quenching related to state transitions; QA, primary quinone acceptor of photosystem II; ΔpH, transthylakoid pH gradient; ΦPSII, quantum yield of photosystem II photochemistry.

Journal Article.  7857 words.  Illustrated.

Subjects: Plant Sciences and Forestry

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