Journal Article

A new FISH protocol with increased sensitivity for physical mapping with short probes in plants

Flavia Guzzo, Evelyn Campagnari and Marisa Levi

in Journal of Experimental Botany

Volume 51, issue 346, pages 965-970
Published in print May 2000 | ISSN: 0022-0957
Published online May 2000 | e-ISSN: 1460-2431 | DOI: https://dx.doi.org/10.1093/jexbot/51.346.965
A new FISH protocol with increased sensitivity for physical mapping with short probes in plants

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Fluorescence in situ hybridization (FISH) is a well‐established technique used for the detection of specific DNA regions, that has been applied to interphase nuclei, pachytene and metaphase chromosomes as well as to extended DNA fibres. This technique allows the physical mapping of specific DNA sequences both on individual chromosomes and extended fibres. A new FISH protocol is described here that enhances the sensitivity of the method. Probes for small unique DNA sequences of less than 2 kb give high signal‐to‐noise ratio with this method, and can be visualized easily by means of conventional fluores cence microscopy.

Keywords: FISH; Asparagus officinalis; physical mapping.

Journal Article.  3969 words.  Illustrated.

Subjects: Plant Sciences and Forestry

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