Journal Article

Leaf age‐dependent differences in sulphur assimilation and allocation in poplar (<i>Populus tremula</i> × <i>P. alba</i>) leaves

Tanja Hartmann, Susanne Mult, Marianne Suter, Heinz Rennenberg and Cornelia Herschbach

in Journal of Experimental Botany

Volume 51, issue 347, pages 1077-1088
Published in print June 2000 | ISSN: 0022-0957
Published online June 2000 | e-ISSN: 1460-2431 | DOI: http://dx.doi.org/10.1093/jexbot/51.347.1077
Leaf age‐dependent differences in sulphur assimilation and allocation in poplar (Populus tremula × P. alba) leaves

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35S‐sulphate was flap‐fed to poplar leaves of different leaf development stages – young developing, expanding, mature, and old mature poplar leaves. 35S‐sulphate was taken up independent of the leaf development stage. Whereas young development leaves did not export the 35S taken up, export increased with increasing leaf development stage. Expanding leaves allocated the exported 35S mainly into apical tree parts (73–87%) and only to a minor extent (13–27%) in basipetal direction. Neither lower trunk sections nor the roots were sinks for the exported 35S. Expanding and developing leaves, but not the shoot apex, were the main sinks for the 35S allocated in apical direction. In contrast, mature and old mature leaves exported the 35S taken up mainly in basipetal direction (65–82%) with the roots constituting the main sinks. The 35S allocated into apical tree parts was found in expanding and developing leaves, but only to a minor extent in the shoot apex. Apical allocated 35S was identified as sulphate. Apparently the demand of young developing leaves for reduced sulphur was not fulfilled by mature leaves. Therefore, reduced sulphur for growth and development of young developing leaves must be supplied from other sources.

In vitro activity of enzymes involved in assimilatory sulphate reduction was measured to investigate whether demand for reduced sulphur by young leaves is met by their own sulphate reduction. ATP sulphurylase and APS reductase activities were not significantly lower in developing than in mature leaves. Sulphite reductase and serine acetyltransferase activities were highest in developing leaves; O‐acetylserine (thiol) lyase activity was similar in all leaf developing stages. Apparently, young developing poplar leaves are able to produce their own reduced sulphur for growth and development. Whether other sources such as storage tissues and/or roots are involved in reduced sulphur supply to developing leaves remains to be elucidated.

Keywords: Poplar trees; glutathione; sulphate; sulphur nutrition; APS reductase; ATP sulphurylase; serine acetyltransferase; O‐acety‐l‐serine (thiol) lyase; sulphite reductase; long‐distance transport; leaf development.; CHES, 2‐(cyclohexylamino)‐ethansulphonacid; DTNB, 5,5′‐dithiobis(2‐nitrobenzolacid); γ‐EC, γ‐glutamylcysteine; mBBr, monobromobimane; OAS, O‐acetylserine; PVPP, polyvinylpolypyrrolidone; PVP, polyvinylpyrrolidone.

Journal Article.  6931 words.  Illustrated.

Subjects: Plant Sciences and Forestry

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