Journal Article

Isolation of cDNA clones corresponding to genes expressed during fruit ripening in Japanese pear (<i>Pyrus pyrifolia</i> Nakai): involvement of the ethylene signal transduction pathway in their expression

Akihiro Itai, Kenji Tanabe, Fumio Tamura and Toshiko Tanaka

in Journal of Experimental Botany

Volume 51, issue 347, pages 1163-1166
Published in print June 2000 | ISSN: 0022-0957
Published online June 2000 | e-ISSN: 1460-2431 | DOI: http://dx.doi.org/10.1093/jexbot/51.347.1163
Isolation of cDNA clones corresponding to genes expressed during fruit ripening in Japanese pear (Pyrus pyrifolia Nakai): involvement of the ethylene signal transduction pathway in their expression

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Thirty cDNA clones of genes corresponding to mRNAs up‐regulated during fruit ripening of Japanese pear (Pyrus pyrifolia Nakai cv. Kikusui) were obtained by differential screening of a ripe fruit cDNA library. All of these cDNAs were sequenced and gathered into 11 non‐redundant groups after database searches. These clones represented genes associated with stress responses, protein catabolism or pathogenesis. The accumulation of transcripts of 3 out of 11 genes was inhibited by 1‐methylcyclopropene (MCP), an inhibitor of ethylene action.

Keywords: Japanese pear; Pyrus pyrifolia; fruit ripening; differential screening; 1‐methylcyclopropene (MCP).

Journal Article.  2442 words.  Illustrated.

Subjects: Plant Sciences and Forestry

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