Journal Article

Changes in gene expression in the <i>leafy cotyledon1</i> (<i>lec1</i>) and <i>fusca3</i> (<i>fus3</i>) mutants of <i>Arabidopsis thaliana</i> L.

Carlos M. Vicient, Natacha Bies‐Etheve and Michel Delseny

in Journal of Experimental Botany

Volume 51, issue 347, pages 995-1003
Published in print June 2000 | ISSN: 0022-0957
Published online June 2000 | e-ISSN: 1460-2431 | DOI:
Changes in gene expression in the leafy cotyledon1 (lec1) and fusca3 (fus3) mutants of Arabidopsis thaliana L.

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Arabidopsis thaliana L. leafy cotyledon1 (lec1) and fusca3 (fus3) mutants show multiple phenotypic defects during seed development. In this report the effects of these mutations are examined at the molecular level. The patterns of protein accumulation in lec1 and fus3 seeds are severly altered. In lec1 seeds the steady‐state mRNA levels of several late embryogenesis genes were reduced. Different patterns of expression were observed, indicating the occurrence of several regulatory pathways. The effect of lec1 mutations on the expression of the late‐embryogenesis abundant AtEm1 gene was examined in detail. In lec1‐1 seeds, the AtEm1 gene was expressed at a higher level than in the wild type and earlier in development. The activity of an AtEm1 promoter/βglucuronidase reporter gene construct in transgenic A.thaliana plants was studied. Changes in promoter activity in lec1‐1 with respect to wild‐type seeds were correlated with changes in corresponding mRNA steady‐state levels. fus3‐2 mutation produced similar changes in AtEm1 promoter activity as lec1‐1, which is consistent with the hypothesis that LEC1 and FUS3 might act in the same regulatory pathway. Transgenic analysis using 5′–promoter deletions demonstrated that at least two regions of AtEm1 gene promoter interact with the LEC1‐dependent transcriptional regulatory pathway. In spite of expression of the AtEm1 promoter and accumulation of AtEm1 mRNA, the corresponding Em1 protein does not accumulate in lec1‐1 seeds. The ABA inducibility of the AtEm1 promoter was not affected by the lec1 mutation.

Keywords: abi3; Em genes; gene expression; late embryogenesis; seed development.

Journal Article.  6346 words.  Illustrated.

Subjects: Plant Sciences and Forestry

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