Journal Article

Analysis of bruchid resistance in the wild common bean accession G02771: no evidence for insecticidal activity of arcelin 5

Alain Goossens, Constanza Quintero, Willy Dillen, Riet De Rycke, Jose Flower Valor, Janniek De Clercq, Marc Van Montagu, Cesar Cardona and Geert Angenon

in Journal of Experimental Botany

Volume 51, issue 348, pages 1229-1236
Published in print July 2000 | ISSN: 0022-0957
Published online July 2000 | e-ISSN: 1460-2431 | DOI: http://dx.doi.org/10.1093/jexbot/51.348.1229
Analysis of bruchid resistance in the wild common bean accession G02771: no evidence for insecticidal activity of arcelin 5

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Arcelins are abundant seed storage proteins thought to be implicated in the resistance of wild Phaseolus vulgaris (L.) genotypes against Zabrotes subfasciatus (Boheman), an important storage insect pest of common bean. Here, the insecticidal activity of the arcelin‐5 variant that is present in the highly resistant P. vulgaris accession G02771 was investigated. No correlation could be established between the presence of arcelin 5 and the insecticidal effects observed in G02771 seeds. Insect feeding assays with artificial seeds into which purified arcelin‐5 protein was incorporated and with transgenic P. acutifolius (A. Gray) seeds in which the arcelin‐5 genes were expressed, showed that the presence of arcelin‐5 proteins, even at elevated levels, was not sufficient to achieve adequate resistance against Z. subfasciatus. The same might apply to other arcelin variants. Nevertheless, as resistance is clearly closely linked to the presence of the arcelin‐1 or arcelin‐5 locus, arcelins remain useful markers in breeding programmes aimed at introgressing high levels of resistance to Z. subfasciatus in P. vulgaris cultivars.

Keywords: Arcelin; bruchid resistance; Mexican bean weevil; Phaseolus; Zabrotes subfasciatus.

Journal Article.  6011 words.  Illustrated.

Subjects: Plant Sciences and Forestry

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