Journal Article

Efficient intergeneric fusion of pea (<i>Pisum sativum</i> L.) and grass pea (<i>Lathyrus sativus</i> L.) protoplasts

P. Durieu and S.J. Ochatt

in Journal of Experimental Botany

Volume 51, issue 348, pages 1237-1242
Published in print July 2000 | ISSN: 0022-0957
Published online July 2000 | e-ISSN: 1460-2431 | DOI:
Efficient intergeneric fusion of pea (Pisum sativum L.) and grass pea (Lathyrus sativus L.) protoplasts

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Large numbers of viable protoplasts of pea (Pisum sativum) and grass pea (Lathyrus sativus) were efficiently and reproducibly obtained and, for the first time, fused. Different procedures for fusion were compared, based either on electrofusion (750, 1000, 1250 or 1500 V cm−1), or on the use of macro or micro‐methods with a polyethylene glycol (PEG 6000 or PEG 1540), or a glycine/high pH solution. Over 10% of viable heterokaryons were obtained, with PEG as the most efficient and reproducible agent for protoplast fusion (>20% of viable heterokaryons). Both the division of heterokaryons and the formation of small calluses were observed.

Keywords: Protoplast fusion; Pisum sativum L.; Lathyrus sativus L.; grain legumes.; MES, 2‐N‐morpholinoethanesulphonic acid; NAA, α‐naphthaleneacetic acid; PEG, polyethylene glycol; 2,4‐D, 2,4‐dichlorophenoxyacetic acid.

Journal Article.  3063 words.  Illustrated.

Subjects: Plant Sciences and Forestry

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