Journal Article

B‐type granule containing protrusions and interconnections between amyloplasts in developing wheat endosperm revealed by transmission electron microscopy and GFP expression

Sandra M.J. Langeveld, Ringo van Wijk, Nico Stuurman, Jan W. Kijne and Sylvia de Pater

in Journal of Experimental Botany

Volume 51, issue 349, pages 1357-1361
Published in print August 2000 | ISSN: 0022-0957
Published online August 2000 | e-ISSN: 1460-2431 | DOI: http://dx.doi.org/10.1093/jexbot/51.349.1357
B‐type granule containing protrusions and interconnections between amyloplasts in developing wheat endosperm revealed by transmission electron microscopy and GFP expression

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Starch granules in mature wheat endosperm show a bimodal size distribution. The formation of small starch granules in wheat endosperm cells was studied by transmission electron microscopy (TEM) and confocal laser scanning microscopy (CLSM) after expression and targeting of fluorescent protein into amyloplasts. Both techniques demonstrated the presence of protrusions emanating from A‐type granules‐containing amyloplasts and the presence of B‐type starch granules in these evaginations. Moreover, CLSM recordings demonstrated the interconnection of the amyloplasts by these protrusions, suggesting a possible role of these protrusions in interplastid communication.

Keywords: Amyloplasts; protrusions; B‐type starch granules; CLSM; wheat endosperm.; CLSM, confocal laser scanning microscopy; DPA, days post anthesis; TEM, transmission electron microscopy; GFP, green fluorescent protein; LM, light microscopy; TP, transit peptide; YFP, yellow fluorescent protein.

Journal Article.  2769 words.  Illustrated.

Subjects: Plant Sciences and Forestry

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