Journal Article

Development of β‐1,3‐glucanase activity in germinated tomato seeds

Yukio Morohashi and Hisashi Matsushima

in Journal of Experimental Botany

Volume 51, issue 349, pages 1381-1387
Published in print August 2000 | ISSN: 0022-0957
Published online August 2000 | e-ISSN: 1460-2431 | DOI: http://dx.doi.org/10.1093/jexbot/51.349.1381
Development of β‐1,3‐glucanase activity in germinated tomato seeds

Show Summary Details

Preview

Laminarin‐hydrolysing activity developed in the endosperm of tomato (Lycopersicon esculentum) seeds following germination. The enzyme was basic (pI>10) and the apparent molecular mass was estimated to be 35 kDa by SDS‐PAGE. It was specific for linear β‐1,3‐glucan substrates. Laminarin was hydrolysed by the enzyme to yield a mixture of oligoglucosides, indicating that the enzyme had an endo‐action pattern. Thus, the enzyme was identified as β‐1,3‐ endoglucanase (EC 3.2.1.39). The activity of the enzyme developed in the endosperm after radicle protrusion (germination) had occurred and the enzyme activity was localized exclusively in the micropylar region of the endosperm where the radicle had penetrated. When the lateral endosperm region, where no induction of the enzyme occurred, was wounded (cut or punctured), there was a marked enhancement of β‐1,3‐glucanase activity. Thus the post‐germinative β‐1,3‐glucanase activity in the micropylar endosperm portion might be brought about by wounding resulting from endosperm rupture by radicle penetration.

Keywords: Endosperm; β‐1,3‐glucanase; germination; tomato seed.

Journal Article.  4936 words.  Illustrated.

Subjects: Plant Sciences and Forestry

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.