Journal Article

Transport of amino acids (<span class="smallCaps">l</span>‐valine, <span class="smallCaps">l</span>‐lysine, <span class="smallCaps">l</span>‐glutamic acid) and sucrose into plasma membrane vesicles isolated from cotyledons of developing pea seeds

A. de Jong and A.C. Borstlap

in Journal of Experimental Botany

Published on behalf of Society for Experimental Biology

Volume 51, issue 351, pages 1663-1670
Published in print October 2000 | ISSN: 0022-0957
Published online October 2000 | e-ISSN: 1460-2431 | DOI: https://dx.doi.org/10.1093/jexbot/51.351.1663
Transport of amino acids (l‐valine, l‐lysine, l‐glutamic acid) and sucrose into plasma membrane vesicles isolated from cotyledons of developing pea seeds

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Transport of the amino acids l‐valine, l‐lysine, and l‐glutamic acid and of sucrose was studied in plasma membrane vesicles isolated from developing cotyledons of pea (Pisum sativum L. cv. Marzia). The vesicles were obtained by aqueous polymer two‐phase partitioning of a microsomal fraction and the uptake was determined after the imposition of a H+‐gradient (ΔpH, inside alkaline) and/or an electrical gradient (Δψ, inside negative) across the vesicle membrane. In the absence of gradients, a distinct, time‐dependent uptake of l‐valine was measured, which could be enhanced about 2‐fold by the imposition of ΔpH. The imposition of Δψ stimulated the influx of valine by 20%, both in the absence and in the presence of ΔpH. Uptake of l‐lysine was more strongly stimulated by Δψ than by ΔpH, and its ΔpH‐dependent uptake was enhanced about 6‐fold by the simultaneous imposition of Δψ. In the absence of gradients the uptake of l‐glutamic acid was about 2‐fold higher than that of l‐valine, but it was not detectably affected by ΔpH or Δψ. Although the transport of sucrose was very low, a stimulating effect of ΔpH could be clearly demonstrated. The results lend further support to the contention that during seed development cotyledonary cells employ H+‐symporters for the active uptake of sucrose and amino acids.

Keywords: Amino acids; cotyledons; plasma membrane; proton symport; sucrose.

Journal Article.  5663 words.  Illustrated.

Subjects: Plant Sciences and Forestry

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