Journal Article

Analysis of cell division parameters and cell cycle gene expression during the cultivation of <i>Arabidopsis thaliana</i> cell suspensions

Caroline Richard, Christine Granier, Dirk Inzé and Lieven De Veylder

in Journal of Experimental Botany

Volume 52, issue 361, pages 1625-1633
Published in print August 2001 | ISSN: 0022-0957
Published online August 2001 | e-ISSN: 1460-2431 | DOI: https://dx.doi.org/10.1093/jexbot/52.361.1625
Analysis of cell division parameters and cell cycle gene expression during the cultivation of Arabidopsis thaliana cell suspensions

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Arabidopsis thaliana cell suspension cultures were characterized for the first time in detail in terms of biomass accumulation, cell division rate and cell cycle phase durations. Subsequently, this model system was used to follow the transcription profile of key cell cycle genes during a complete cultivation cycle. According to the calculated changes in the relative division rate over time, the cell cycle genes could be classified into four groups based on their transcriptional expression pattern. These differential patterns of gene expression are discussed with respect to the putative roles of the different cell cycle genes in the division cycle. Analysis of protein levels showed that mRNA levels did not correlate with protein levels in all cases. Results obtained in other systems, such as BY‐2 cell suspensions or plants, confirm that cell suspension cultures of A. thaliana are suitable for the analysis of cell cycle regulation.

Keywords: Arabidopsis thaliana; cell cycle; gene expression.

Journal Article.  5148 words.  Illustrated.

Subjects: Plant Sciences and Forestry

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