Journal Article

Hypoxia-inducible Factor 1α and Antiangiogenic Activity of Farnesyltransferase Inhibitor SCH66336 in Human Aerodigestive Tract Cancer

Ji-Youn Han, Seung Hyun Oh, Floriana Morgillo, Jeffrey N. Myers, Edward Kim, Waun Ki Hong and Ho-Young Lee

in JNCI: Journal of the National Cancer Institute

Volume 97, issue 17, pages 1272-1286
Published in print September 2005 | ISSN: 0027-8874
Published online September 2005 | e-ISSN: 1460-2105 | DOI: http://dx.doi.org/10.1093/jnci/dji251
Hypoxia-inducible Factor 1α and Antiangiogenic Activity of Farnesyltransferase Inhibitor SCH66336 in Human Aerodigestive Tract Cancer

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Background: The farnesyltransferase inhibitor SCH66336, in combination with other receptor tyrosine kinase inhibitors, inhibits the growth of non–small-cell lung cancer (NSCLC) cells. We examined whether SCH66336 inhibits angiogenesis of aerodigestive tract cancer cells. Methods: Antiangiogenic activities of SCH66336 against NSCLC, head and neck squamous cell carcinoma (HNSCC), and endothelial cells were examined with cell proliferation, capillary tube formation, and chick aorta (under hypoxic, normoxic, insulin-like growth factor I (IGF)–stimulated, and unstimulated conditions); reverse transcription–polymerase chain reaction; and western blot analyses. The specific roles of the ubiquitin-mediated proteasome machinery, mitogen-activated protein kinase (MAPK) and Akt pathways, and heat shock protein 90 (Hsp90) in the SCH66336-mediated degradation of hypoxia-inducible factor 1α (HIF-1α) were assessed with ubiquitin inhibitors and adenoviral vectors that express constitutively active MAP kinase kinase (MEK)1, constitutively active Akt, or Hsp90. Results: SCH66336 showed antiangiogenic activities and decreased the expression of vascular endothelial cell growth factor (VEGF) and HIF-1α in hypoxic, IGF-stimulated, and unstimulated aerodigestive tract cancer and endothelial cells. SCH66336 reduced the half-life of the HIF-1α protein, and ubiquitin inhibitors protected the hypoxia- or IGF-stimulated HIF-1α protein from SCH66336-mediated degradation. SCH66336 inhibited the interaction between HIF-1α and Hsp90. The overexpression of Hsp90, but not constitutive Akt or constitutive MEK, restored HIF-1α expression in IGF-stimulated or hypoxic cells but not in unstimulated cells. Conclusions: SCH66336 appears to inhibit angiogenic activities of NSCLC and HNSCC cells by decreasing hypoxia- or IGF-stimulated HIF-1α expression and to inhibit VEGF production by inhibiting the interaction between HIF-1α and Hsp90, resulting in the proteasomal degradation of HIF-1α.

Journal Article.  12521 words.  Illustrated.

Subjects: Medical Oncology

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