Journal Article

Abundance of BER-related proteins depends on cell proliferation status and the presence of DNA polymerase β

Mizuki Yamamoto, Ryohei Yamamoto, Shigeo Takenaka, Satoshi Matsuyama and Kihei Kubo

in Journal of Radiation Research

Volume 56, issue 4, pages 607-614
Published in print July 2015 | ISSN: 0449-3060
Published online March 2015 | e-ISSN: 1349-9157 | DOI: http://dx.doi.org/10.1093/jrr/rrv010

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  • Clinical Genetics
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In mammalian cells, murine N-methylpurine DNA glycosylase (MPG) removes bases damaged spontaneously or by chemical agents through the process called base excision repair (BER). In this study, we investigated the influence of POL β deficiency on MPG-initiated BER efficiency and the expression levels of BER-related proteins in log-phase and growth-arrested (G0) mouse embryonic fibroblasts (MEFs). G0 wild-type (WT) or POL β–deficient (Pol β–KO) cells showed greater resistance to methyl methanesulfonate than did log-phase cells, and repair of methylated bases was less efficient in the G0 cells. Apex1 mRNA expression was significantly lower in Pol β–KO or G0 WT MEFs than in log-phase WT MEFs. Moreover, although Mpg mRNA levels did not differ significantly among cell types, MPG protein levels were significantly higher in log-phase WT cells than in log-phase Pol β–KO cells or either type of G0 cells. Additionally, proliferating cell nuclear antigen protein levels were also reduced in log-phase Pol β–KO cells or either type of G0 cells. These results indicated that MPG-initiated BER functions mainly in proliferating cells, but less so in G0 cells, and that POL β may be involved in regulation of the amount of intracellular repair proteins.

Keywords: BER; MPG; mouse; cell proliferation; POL β

Journal Article.  4559 words.  Illustrated.

Subjects: Clinical Genetics ; Molecular Biology and Genetics ; Epidemiology ; Radiology ; Nuclear Chemistry, Photochemistry, and Radiation

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