Journal Article

Infection process and the interaction of rice roots with rhizobia

Francine M. Perrine-Walker, Joko Prayitno, Barry G. Rolfe, Jeremy J. Weinman and Charles H. Hocart

in Journal of Experimental Botany

Published on behalf of Society for Experimental Biology

Volume 58, issue 12, pages 3343-3350
Published in print September 2007 | ISSN: 0022-0957
Published online October 2007 | e-ISSN: 1460-2431 | DOI: https://dx.doi.org/10.1093/jxb/erm181

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Most rhizobial strains inhibit rice root growth in the presence of calcium or potassium nitrates, but not ammonium nitrate. Certain rhizobial strains, however, such as strain R4, do not inhibit rice growth and can enter rice roots and multiply in the intercellular spaces. By using the green fluorescent protein (GFP) as a visual marker, it was found that Rhizobium became intimately associated with rice seedling roots within 24–48 h. During this initial period it was observed that strain R4 could cause structural changes resembling infection threads within the rice root hairs. Generally, the sites of the emerging lateral roots provide a temporary entry point for rhizobia, either by root hair entry or crack entry. All tested GFP-labelled Rhizobium strains infected the root hairs near the base of growing lateral roots. This study suggests that some strains may have the ability to infect rice root tissues via root hairs located at the emerging lateral roots and to spread extensively throughout the rice root.

Keywords: Green fluorescent protein; infection; non-legumes; Rhizobium; Rhizobium–rice association; rice growth inhibition; short lateral roots

Journal Article.  4883 words.  Illustrated.

Subjects: Plant Sciences and Forestry

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