Journal Article

Identification and characterization of carrot HAP factors that form a complex with the embryo-specific transcription factor C-LEC1

Katsumi Yazawa and Hiroshi Kamada

in Journal of Experimental Botany

Published on behalf of Society for Experimental Biology

Volume 58, issue 13, pages 3819-3828
Published in print October 2007 | ISSN: 0022-0957
Published online October 2007 | e-ISSN: 1460-2431 | DOI: https://dx.doi.org/10.1093/jxb/erm238

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C-LEC1, an orthologue of Arabidopsis LEC1, is thought to be an essential transcriptional activator required for normal development during the early and late phases of embryogenesis. C-LEC1 is similar in sequence to the HAP3 subunits of other organisms. To understand C-LEC1 function better, a cDNA library of carrot somatic embryos was screened for factors that form complexes with C-LEC1. Two carrot HAP5 homologues and two carrot HAP2 homologues were identified; these factors have significant sequence similarity to the conserved regions of HAP5 and HAP2, respectively. Some of these proteins form heterotrimeric complexes that bind specifically to DNA fragments containing a CCAAT sequence in vitro. The results suggest that C-LEC1 is a component of the CCAAT-box-binding factor and forms a complex with C-HAP2B and C-HAP5A or C-HAP5B that regulates gene expression during carrot embryo development.

Keywords: Carrot; CCAAT-box; C-HAP; C-LEC1; Daucus carota; DNA binding; embryogenesis

Journal Article.  5257 words.  Illustrated.

Subjects: Plant Sciences and Forestry

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