Journal Article

Jasmonate-induced transcriptional changes suggest a negative interference with the ripening syndrome in peach fruit

Vanina Ziosi, Claudio Bonghi, Anna Maria Bregoli, Livio Trainotti, Stefania Biondi, Setha Sutthiwal, Satoru Kondo, Guglielmo Costa and Patrizia Torrigiani

in Journal of Experimental Botany

Published on behalf of Society for Experimental Biology

Volume 59, issue 3, pages 563-573
Published in print February 2008 | ISSN: 0022-0957
Published online February 2008 | e-ISSN: 1460-2431 | DOI: http://dx.doi.org/10.1093/jxb/erm331

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Peach (Prunus persica L. Batsch) was chosen as a model to shed light on the physiological role of jasmonates (JAs) during fruit ripening. To this aim, the effects of methyl jasmonate (MJ, 0.40 mM) and propyl dihydrojasmonate (PDJ, 0.22 mM), applied in planta at different fruit developmental stages, on the time-course of ethylene production and fruit quality traits were evaluated. MJ-induced changes in fruit transcriptome at harvest and the expression profiling of relevant JA-responsive genes were analysed in control and JA-treated fruit. Exogenously applied JAs affected the onset of ripening depending upon the fruit developmental stage, with PDJ being more active than MJ. Both compounds enhanced the transcription of allene oxide synthase (PpAOS1), the first specific enzyme in the biosynthesis of jasmonic acid, and altered the pattern of jasmonic acid accumulation. Microarray transcriptome profiling showed that MJ down-regulated some ripening-related genes, such as 1-aminocyclopropane-1-carboxylic acid oxidase (PpACO1) and polygalacturonase (PG), and the transcriptional modulator IAA7. MJ also altered the expression of cell wall-related genes, namely pectate lyase (PL) and expansins (EXPs), and up-regulated several stress-related genes, including some of those involved in JA biosynthesis. Time-course expression profiles of PpACO1, PL, PG, PpExp1, and the transcription factor LIM confirmed the array results. Thus, in peach fruit, exogenous JAs led to a ripening delay due to an interference with ripening- and stress/defence-related genes, as reflected in the transcriptome of treated fruit at harvest.

Keywords: Allene oxide synthase; ethylene; fruit ripening; jasmonic acid; methyl jasmonate; microarray; propyl dihydrojasmonate; Prunus persica

Journal Article.  6182 words.  Illustrated.

Subjects: Plant Sciences and Forestry

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