Journal Article

Glycosylation of S-RNases may influence pollen rejection thresholds in <i>Solanum chacoense</i>

Bolin Liu, David Morse and Mario Cappadocia

in Journal of Experimental Botany

Published on behalf of Society for Experimental Biology

Volume 59, issue 3, pages 545-552
Published in print February 2008 | ISSN: 0022-0957
Published online February 2008 | e-ISSN: 1460-2431 | DOI: https://dx.doi.org/10.1093/jxb/erm339

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A survey of Solanum chacoense plants expressing an authentic S11-RNase transgene identified a line with partial compatibility to S11 pollen. By comparing fruit set to the S-RNase levels determined immunologically in single styles, the minimum level of S11-RNase required for full rejection of S11 pollen was estimated to be 18 ng per style. The S11-RNase threshold levels are thus considerably lower than those previously reported for the S12-RNase. Interestingly, these two allelic S-RNases differ dramatically in the extent of glycosylation, with the number of glycosylation sites varying from one (S11-RNase) to four (S12-RNase). It is suggested that reduced glycosylation of the S11-RNase may be related to the lower threshold for pollen rejection.

Keywords: Gametophytic self-incompatibility; glycosylation; pistil-by-pistil analysis; S-RNase; Solanum chacoense; threshold

Journal Article.  4587 words.  Illustrated.

Subjects: Plant Sciences and Forestry

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