Journal Article

Plant response to nitrate starvation is determined by N storage capacity matched by nitrate uptake capacity in two <i>Arabidopsis</i> genotypes

Céline Richard-Molard, Anne Krapp, François Brun, Bertrand Ney, Françoise Daniel-Vedele and Sylvain Chaillou

in Journal of Experimental Botany

Published on behalf of Society for Experimental Biology

Volume 59, issue 4, pages 779-791
Published in print March 2008 | ISSN: 0022-0957
Published online February 2008 | e-ISSN: 1460-2431 | DOI: https://dx.doi.org/10.1093/jxb/erm363
Plant response to nitrate starvation is determined by N storage capacity matched by nitrate uptake capacity in two Arabidopsis genotypes

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In a low-input agricultural context, plants facing temporal nutrient deficiencies need to be efficient. By comparing the effects of NO3-starvation in two lines of Arabidopsis thaliana (RIL282 and 432 from the Bay-0×Shahdara population), this study aimed to screen the physiological mechanisms allowing one genotype to withstand NO3-deprivation better than another and to rate the relative importance of processes such as nitrate uptake, storage, and recycling. These two lines, chosen because of their contrasted shoot N contents for identical shoot biomass under N-replete conditions, underwent a 10 d nitrate starvation after 28 d of culture at 5 mM NO3. It was demonstrated that line 432 coped better with NO3-starvation, producing higher shoot and root biomass and sustaining maximal growth for a longer time. However, both lines exhibited similar features under NO3-starvation conditions. In particular, the nitrate pool underwent the same drastic and early depletion, whereas the protein pool was increased to a similar extent. Nitrate remobilization rate was identical too. It was proportional to nitrate content in both shoots and roots, but it was higher in roots. One difference emerged: line 432 had a higher nitrate content at the beginning of the starvation phase. This suggests that to overcome NO3-starvation, line 432 did not directly rely on the N pool composition, nor on nitrate remobilization efficiency, but on higher nitrate storage capacities prior to NO3-starvation. Moreover, the higher resistance of 432 corresponded to a higher nitrate uptake capacity and a 2–9-fold higher expression of AtNRT1.1, AtNRT2.1, and AtNRT2.4 genes, suggesting that the corresponding nitrate transporters may be preferentially involved under fluctuating N supply conditions.

Keywords: Arabidopsis thaliana; genetic variability; N partitioning; N recycling; N use efficiency; nitrate deficiency; nitrate remobilization rate; nitrate transporter gene expression; nitrogen reserves; plant development

Journal Article.  7477 words.  Illustrated.

Subjects: Plant Sciences and Forestry

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