Journal Article

Hydrogen peroxide generated by copper amine oxidase is involved in abscisic acid-induced stomatal closure in <i>Vicia faba</i>

Zhenfeng An, Wen Jing, Youliang Liu and Wenhua Zhang

in Journal of Experimental Botany

Published on behalf of Society for Experimental Biology

Volume 59, issue 4, pages 815-825
Published in print March 2008 | ISSN: 0022-0957
Published online February 2008 | e-ISSN: 1460-2431 | DOI: http://dx.doi.org/10.1093/jxb/erm370
Hydrogen peroxide generated by copper amine oxidase is involved in abscisic acid-induced stomatal closure in Vicia faba

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H2O2 is an essential signal in absicic acid (ABA)-induced stomatal closure. It can be synthesized by several enzymes in plants. In this study, the roles of copper amine oxidase (CuAO) in H2O2 production and stomatal closure were investigated. Exogenous ABA stimulated apoplast CuAO activity, increased H2O2 production and [Ca2+]cyt levels in Vicia faba guard cells, and induced stomatal closure. These processes were impaired by CuAO inhibitor(s). In the metabolized products of CuAO, only H2O2 could induce stomatal closure. By the analysis of enzyme kinetics and polyamine contents in leaves, putrescine was regarded as a substrate of CuAO. Putrescine showed similar effects with ABA on the regulation of H2O2 production, [Ca2+]cyt levels, as well as stomatal closure. The results suggest that CuAO in V. faba guard cells is an essential enzymatic source for H2O2 production in ABA-induced stomatal closure via the degradation of putrescine. Calcium messenger is an important intermediate in this process.

Keywords: Abscisic acid; calcium; copper amine oxidase; hydrogen peroxide; putrescine; stomatal closure; Vicia faba

Journal Article.  6353 words.  Illustrated.

Subjects: Plant Sciences and Forestry

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