Journal Article

Development of abiotic stress tolerance via bZIP-type transcription factor LIP19 in common wheat

Fuminori Kobayashi, Eri Maeta, Akihiro Terashima, Kanako Kawaura, Yasunari Ogihara and Shigeo Takumi

in Journal of Experimental Botany

Published on behalf of Society for Experimental Biology

Volume 59, issue 4, pages 891-905
Published in print March 2008 | ISSN: 0022-0957
Published online March 2008 | e-ISSN: 1460-2431 | DOI: https://dx.doi.org/10.1093/jxb/ern014

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Cereal lip19 genes encoding bZIP-type transcription factors are assumed to play a regulatory role in gene expression during the cold acclimation process. However, no direct evidence shows an association of LIP19-type bZIPs with stress tolerance or activation of stress-responsive Cor/Lea genes. To understand the molecular basis of development of abiotic stress tolerance through the LIP19 transcription factor, a wheat lip19 homologue, Wlip19, was isolated and characterized. Wlip19 expression was activated by low temperature in seedlings and was higher in a freezing-tolerant cultivar than in a freezing-sensitive one. Wlip19 also responded to drought and exogenous ABA treatment. Wlip19-expressing transgenic tobacco showed a significant increase in abiotic stress tolerance, especially freezing tolerance. Expression of a GUS reporter gene under the control of promoter sequences of four wheat Cor/Lea genes, Wdhn13, Wrab17, Wrab18, and Wrab19, was enhanced by Wlip19 expression in wheat callus and tobacco plants. These results indicate that WLIP19 acts as a transcriptional regulator of Cor/Lea genes in the development of abiotic stress tolerance. Moreover, direct protein–protein interaction between WLIP19 and a wheat OBF1 homologue TaOBF1, another bZIP-type transcription factor, was observed, suggesting that this interaction is conserved in cereals.

Keywords: Abiotic stress tolerance; bZIP protein; Cor/Lea genes; transgenic plants; Triticum aestivum L

Journal Article.  6993 words.  Illustrated.

Subjects: Plant Sciences and Forestry

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