Journal Article

Elicitor-dependent expression of the ribosome-inactivating protein beetin is developmentally regulated

Rosario Iglesias, Yolanda Pérez, Lucía Citores, José M. Ferreras, Enrique Méndez and Tomás Girbés

in Journal of Experimental Botany

Published on behalf of Society for Experimental Biology

Volume 59, issue 6, pages 1215-1223
Published in print April 2008 | ISSN: 0022-0957
Published online March 2008 | e-ISSN: 1460-2431 | DOI: http://dx.doi.org/10.1093/jxb/ern030
Elicitor-dependent expression of the ribosome-inactivating protein beetin is developmentally regulated

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BE27 and BE29 are two forms of beetin, a virus-inducible type 1 ribosome-inactivating protein isolated from leaves of Beta vulgaris L. Western blot analysis revealed the presence of beetin forms in adult plants but not in germ or young plants, indicating that the expression of these proteins is developmentally regulated. While beetins are expressed only in adult plants, their transcripts are present through all stages of development. In addition, the treatment of B. vulgaris leaves with mediators of plant-acquired resistance such as salicylic acid and hydrogen peroxide promoted the expression of beetin by induction of its transcript, but only in adult plants. The plant expresses three mRNAs which differ only in their 3′ untranslated region. All these observations suggest a dual regulation of beetin expression, i.e. at the post-transcriptional and transcriptional levels. Additionally, total RNA isolated from leaves treated with hydrogen peroxide, which express high levels of active beetin, is not de-adenylated by endogenous beetin, nor in vitro by the addition of BE27, thus suggesting that sugar beet ribosomes are resistant to beetin.

Keywords: Beetin; Beta vulgaris; hydrogen peroxide; ribosome-inactivating protein (RIP); salicylic acid; 3′ UTR

Journal Article.  5725 words.  Illustrated.

Subjects: Plant Sciences and Forestry

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