Journal Article

Immunocytochemical localization of <i>Pisum sativum</i> TRXs <i>f</i> and <i>m</i> in non-photosynthetic tissues

José A. Traverso, Florence Vignols, Roland Cazalis, Antonio J. Serrato, Pablo Pulido, Mariam Sahrawy, Yves Meyer, Francisco Javier Cejudo and Ana Chueca

in Journal of Experimental Botany

Published on behalf of Society for Experimental Biology

Volume 59, issue 6, pages 1267-1277
Published in print April 2008 | ISSN: 0022-0957
Published online March 2008 | e-ISSN: 1460-2431 | DOI: http://dx.doi.org/10.1093/jxb/ern037

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Plants are the organisms containing the most complex multigenic family for thioredoxins (TRX). Several types of TRXs are targeted to chloroplasts, which have been classified into four subgroups: m, f, x, and y. Among them, TRXs f and m were the first plastidial TRXs characterized, and their function as redox modulators of enzymes involved in carbon assimilation in the chloroplast has been well-established. Both TRXs, f and m, were named according to their ability to reduce plastidial fructose-1,6-bisphosphatase (FBPase) and malate dehydrogenase (MDH), respectively. Evidence is presented here based on the immunocytochemistry of the localization of f and m-type TRXs from Pisum sativum in non-photosynthetic tissues. Both TRXs showed a different spatial pattern. Whilst PsTRXm was localized to vascular tissues of all the organs analysed (leaves, stems, and roots), PsTRXf was localized to more specific cells next to xylem vessels and vascular cambium. Heterologous complementation analysis of the yeast mutant EMY63, deficient in both yeast TRXs, by the pea plastidial TRXs suggests that PsTRXm, but not PsTRXf, is involved in the mechanism of reactive oxygen species (ROS) detoxification. In agreement with this function, the PsTRXm gene was induced in roots of pea plants in response to hydrogen peroxide.

Keywords: Heterologous complementation; oxidative stress; pea; Pisum sativum; thioredoxin; vascular tissue; yeast EMY63

Journal Article.  6045 words.  Illustrated.

Subjects: Plant Sciences and Forestry

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