Journal Article

Physiological and molecular adaptations to drought in Andean potato genotypes

Cecilia Vasquez-Robinet, Shrinivasrao P. Mane, Alexander V. Ulanov, Jonathan I. Watkinson, Verlyn K. Stromberg, David De Koeyer, Roland Schafleitner, David B. Willmot, Merideth Bonierbale, Hans J. Bohnert and Ruth Grene

in Journal of Experimental Botany

Published on behalf of Society for Experimental Biology

Volume 59, issue 8, pages 2109-2123
Published in print May 2008 | ISSN: 0022-0957
Published online May 2008 | e-ISSN: 1460-2431 | DOI: http://dx.doi.org/10.1093/jxb/ern073

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The drought stress tolerance of two Solanum tuberosum subsp. andigena landraces, one hybrid (adg×tbr) and Atlantic (S. tuberosum subsp. tuberosum) has been evaluated. Photosynthesis in the Andigena landraces during prolonged drought was maintained significantly longer than in the Tuberosum (Atlantic) line. Among the Andigena landraces, ‘Sullu’ (SUL) was more drought resistant than ‘Negra Ojosa’ (NOJ). Microarray analysis and metabolite data from leaf samples taken at the point of maximum stress suggested higher mitochondrial metabolic activity in SUL than in NOJ. A greater induction of chloroplast-localized antioxidant and chaperone genes in SUL compared with NOJ was evident. ABA-responsive TFs were more induced in NOJ compared with SUL, including WRKY1, mediating a response in SA signalling that may give rise to increased ROS. NOJ may be experiencing higher ROS levels than SUL. Metabolite profiles of NOJ were characterized by compounds indicative of stress, for example, proline, trehalose, and GABA, which accumulated to a higher degree than in SUL. The differences between the Andigena lines were not explained by protective roles of compatible solutes; hexoses and complex sugars were similar in both landraces. Instead, lower levels of ROS accumulation, greater mitochondrial activity and active chloroplast defences contributed to a lower stress load in SUL than in NOJ during drought.

Keywords: Chaperones; drought; flavonoids; metabolites; oligonucleotide-based microarray; osmolytes; potato; ROS; subspecies Andigena

Journal Article.  8477 words.  Illustrated.

Subjects: Plant Sciences and Forestry

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