Journal Article

Expression of ethylene biosynthetic and receptor genes in rose floral tissues during ethylene-enhanced flower opening

Jingqi Xue, Yunhui Li, Hui Tan, Feng Yang, Nan Ma and Junping Gao

in Journal of Experimental Botany

Published on behalf of Society for Experimental Biology

Volume 59, issue 8, pages 2161-2169
Published in print May 2008 | ISSN: 0022-0957
Published online May 2008 | e-ISSN: 1460-2431 | DOI:

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Ethylene production, as well as the expression of ethylene biosynthetic (Rh-ACS14 and Rh-ACO1) and receptor (Rh-ETR1–5) genes, was determined in five different floral tissues (sepals, petals, stamens, gynoecia, and receptacles) of cut rose (Rosa hybrida cv. Samantha upon treatment with ethylene or the ethylene inhibitor 1-methylcyclopropene (1-MCP). Ethylene-enhanced ethylene production occurred only in gynoecia, petals, and receptacles, with gynoecia showing the greatest enhancement in the early stage of ethylene treatment. However, 1-MCP did not suppress ethylene production in these three tissues. In sepals, ethylene production was highly decreased by ethylene treatment, and increased dramatically by 1-MCP. Ethylene production in stamens remained unchanged after ethylene or 1-MCP treatment. Induction of certain ethylene biosynthetic genes by ethylene in different floral tissues was positively correlated with the ethylene production, and this induction was also not suppressed by 1-MCP. The expression of Rh-ACS2 and Rh-ACS3 was quickly induced by ethylene in gynoecia, but neither Rh-ACS1 nor Rh-ACS4 was induced by ethylene in any of the five tissues. In addition, Rh-ACO1 was induced by ethylene in all floral tissues except sepals. The induced expression of ethylene receptor genes by ethylene was much faster in gynoecia than in petals, and the expression of Rh-ETR3 was strongly suppressed by 1-MCP in all floral tissues. These results indicate that ethylene biosynthesis in gynoecia is regulated developmentally, rather than autocatalytically. The response of rose flowers to ethylene occurs initially in gynoecia, and ethylene may regulate flower opening mainly through the Rh-ETR3 gene in gynoecia.

Keywords: Cut roses; ethylene biosynthesis; ethylene receptor; floral tissues; flower opening; gene expression; Rosa hybrida

Journal Article.  4717 words.  Illustrated.

Subjects: Plant Sciences and Forestry

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