Journal Article

Rice–arsenate interactions in hydroponics: whole genome transcriptional analysis

Gareth J. Norton, Daniel E. Lou-Hing, Andrew A. Meharg and Adam H. Price

in Journal of Experimental Botany

Published on behalf of Society for Experimental Biology

Volume 59, issue 8, pages 2267-2276
Published in print May 2008 | ISSN: 0022-0957
Published online May 2008 | e-ISSN: 1460-2431 | DOI: http://dx.doi.org/10.1093/jxb/ern097

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Rice (Oryza sativa) varieties that are arsenate-tolerant (Bala) and -sensitive (Azucena) were used to conduct a transcriptome analysis of the response of rice seedlings to sodium arsenate (AsV) in hydroponic solution. RNA extracted from the roots of three replicate experiments of plants grown for 1 week in phosphate-free nutrient with or without 13.3 μM AsV was used to challenge the Affymetrix (52K) GeneChip Rice Genome array. A total of 576 probe sets were significantly up-regulated at least 2-fold in both varieties, whereas 622 were down-regulated. Ontological classification is presented. As expected, a large number of transcription factors, stress proteins, and transporters demonstrated differential expression. Striking is the lack of response of classic oxidative stress-responsive genes or phytochelatin synthases/synthatases. However, the large number of responses from genes involved in glutathione synthesis, metabolism, and transport suggests that glutathione conjugation and arsenate methylation may be important biochemical responses to arsenate challenge. In this report, no attempt is made to dissect differences in the response of the tolerant and sensitive variety, but analysis in a companion article will link gene expression to the known tolerance loci available in the Bala×Azucena mapping population.

Keywords: Arsenate tolerance; candidate genes; glutathione-S-transferase; microarray; Oryza sativa

Journal Article.  6137 words.  Illustrated.

Subjects: Plant Sciences and Forestry

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