Journal Article

Identification of auxins by a chemical genomics approach

May Christian, William B. Hannah, Hartwig Lüthen and Alan M. Jones

in Journal of Experimental Botany

Published on behalf of Society for Experimental Biology

Volume 59, issue 10, pages 2757-2767
Published in print July 2008 | ISSN: 0022-0957
Published online May 2008 | e-ISSN: 1460-2431 | DOI: http://dx.doi.org/10.1093/jxb/ern133

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Thirteen auxenic compounds were discovered in a screen of 10 000 compounds for auxin-like activity in Arabidopsis roots. One of the most potent substances was 2-(4-chloro-2-methylphenoxy)-N-(4-H-1,2,4-triazol-3-yl)acetamide (WH7) which shares similar structure to the known auxenic herbicide 2,4-dichlorophenoxyacetic acid (2,4-D). A selected set of 20 analogues of WH7 was used to provide detailed information about the structure–activity relationship based on their efficacy at inhibiting and stimulating root and shoot growth, respectively, and at induction of gene expression. It was shown that WH7 acts in a genetically defined auxin pathway. These small molecules will extend the arsenal of substances that can be used to define auxin perception site(s) and to dissect subsequent signalling events.

Keywords: Auxin; 2,4-D; growth control; sensitivity; structure–activity

Journal Article.  5853 words.  Illustrated.

Subjects: Plant Sciences and Forestry

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