Journal Article

The POK/AtVPS52 protein localizes to several distinct post-Golgi compartments in sporophytic and gametophytic cells

Hélène Guermonprez, Andrei Smertenko, Marie-Thérèse Crosnier, Monique Durandet, Nathalie Vrielynck, Philippe Guerche, Patrick J. Hussey, Béatrice Satiat-Jeunemaitre and Sandrine Bonhomme

in Journal of Experimental Botany

Published on behalf of Society for Experimental Biology

Volume 59, issue 11, pages 3087-3098
Published in print August 2008 | ISSN: 0022-0957
Published online June 2008 | e-ISSN: 1460-2431 | DOI:
The POK/AtVPS52 protein localizes to several distinct post-Golgi compartments in sporophytic and gametophytic cells

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The organization and dynamics of the plant endomembrane system require both universal and plant-specific molecules and compartments. The latter, despite the growing wealth of information, remains poorly understood. From the study of an Arabidopsis thaliana male gametophytic mutant, it was possible to isolate a gene named POKY POLLEN TUBE (POK) essential for pollen tube tip growth. The similarity between the predicted POK protein sequence and yeast Vps52p, a subunit from the GARP/VFT complex which is involved in the docking of vesicles from the prevacuolar compartment to the Golgi apparatus, suggested that the POK protein plays a role in plant membrane trafficking. Genetic analysis of Arabidopsis mutants affecting AtVPS53 or AtVPS54 genes which encode putative POK partners shows a transmission defect through the male gametophyte for all lines, which is similar to the pok mutant. Using a combination of biochemical approaches and specific antiserum it has been demonstrated that the POK protein is present in phylogenetically divergent plant species, associated with membranes and belongs to a high molecular weight complex. Combination of immunolocalization studies and pharmacological approaches in different plant cells revealed that the POK protein associates with Golgi and post-Golgi compartments. The role of POK in post-Golgi endomembrane trafficking and as a member of a putative plant GARP/VFT complex is discussed.

Keywords: Arabidopsis mutants; GARP/VFT complex; Golgi and post-Golgi compartments; pollen; pollen tube

Journal Article.  7128 words.  Illustrated.

Subjects: Plant Sciences and Forestry

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