Journal Article

Genetic and molecular characterization of three novel <i>S</i>-haplotypes in sour cherry (<i>Prunus cerasus</i> L.)

Tatsuya Tsukamoto, Daniel Potter, Ryutaro Tao, Cristina P. Vieira, Jorge Vieira and Amy F. Iezzoni

in Journal of Experimental Botany

Published on behalf of Society for Experimental Biology

Volume 59, issue 11, pages 3169-3185
Published in print August 2008 | ISSN: 0022-0957
Published online July 2008 | e-ISSN: 1460-2431 | DOI: http://dx.doi.org/10.1093/jxb/ern172

Show Summary Details

Preview

Tetraploid sour cherry (Prunus cerasus L.) exhibits gametophytic self-incompatibility (GSI) whereby the specificity of self-pollen rejection is controlled by alleles of the stylar and pollen specificity genes, S-RNase and SFB (S haplotype-specific F-box protein gene), respectively. As sour cherry selections can be either self-compatible (SC) or self-incompatible (SI), polyploidy per se does not result in SC. Instead the genotype-dependent loss of SI in sour cherry is due to the accumulation of non-functional S-haplotypes. The presence of two or more non-functional S-haplotypes within sour cherry 2x pollen renders that pollen SC. Two new S-haplotypes from sour cherry, S33 and S34, that are presumed to be contributed by the P. fruticosa species parent, the complete S-RNase and SFB sequences of a third S-haplotype, S35, plus the presence of two previously identified sweet cherry S-haplotypes, S14 and S16 are described here. Genetic segregation data demonstrated that the S16-, S33-, S34-, and S35-haplotypes present in sour cherry are fully functional. This result is consistent with our previous finding that ‘hetero-allelic’ pollen is incompatible in sour cherry. Phylogenetic analyses of the SFB and S-RNase sequences from available Prunus species reveal that the relationships among S-haplotypes show no correspondence to known organismal relationships at any taxonomic level within Prunus, indicating that polymorphisms at the S-locus have been maintained throughout the evolution of the genus. Furthermore, the phylogenetic relationships among SFB sequences are generally incongruent with those among S-RNase sequences for the same S-haplotypes. Hypotheses compatible with these results are discussed.

Keywords: Prunus cerasus; self-incompatibility; SFB; S-RNase

Journal Article.  9788 words.  Illustrated.

Subjects: Plant Sciences and Forestry

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.